John at 14:08, 14 July 2023

2023-07-14T14:08:18Z

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John: Created page with "---- link=LPL.jpg
2023-07-14T14:04:03Z

Created page with "---- 200px|link=LPL.jpg <span style="color: navy>'''Background to flow cytometric diagnosis'''</span> Cells of LPL share features of mature B-cell differentation and features of early plasma cell differentiation, and have mixed populations with features of each (although proportions vary). This can be a challenging diagnosis by flow cytometry alone, but other modalities can generally make this diagnosis clear. <div style="width: 95%; overflow: a..."

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[[file:LPL.jpg|200px|link=LPL.jpg]]

<span style="color: navy>'''Background to flow cytometric diagnosis'''</span>

Cells of LPL share features of mature B-cell differentation and features of early plasma cell differentiation, and have mixed populations with features of each (although proportions vary). This can be a challenging diagnosis by flow cytometry alone, but other modalities can generally make this diagnosis clear.

<div style="width: 95%; overflow: auto; border: 1px solid navy; font-size:100%">
{| class="wikitable" style="color:black; background-color:#ffffff;" cellpadding="5"
!colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"|'''Immunophenotype of LPL'''
|-
!colspan="4" style = "font-size:90%; background:#DAE8F2"|'''CD45''' expression
|-
|colspan="4" style = "font-size:90%; color:black;" |Typically expression of CD45 is similar to that of normal T and B cells.
|-
!colspan="4" style = "font-size:90%; background:#DAE8F2"|Major markers useful in LPL diagnosis
|-
|colspan="1" style = "font-size:80%; width: 4%; |'''Marker'''
|colspan="1" style = "font-size:80%; width: 8%; |'''Freq'''
|colspan="1" style = "font-size:80%; width: 8%; |'''Level'''
|colspan="1" style = "font-size:80%; width: 80%; |'''Comment'''
|-
|colspan="1" style = "font-size:90%; color:black;" |CD19
|colspan="1" style = "background:#006699;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Generally CD19 will be expressed
|-
|colspan="1" style = "font-size:90%; color:black;" |CD38
|colspan="1" style = "background:#006699;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|
|-
|colspan="1" style = "width: 4%; font-size:90%; color:black;" |κ/λ
|colspan="1" style = "width: 8%; background:#006699;"|
|colspan="1" style = "font-size:80%; width: 8%; |
|colspan="1" style = "font-size:80%;"|Light chain retriction usually detected
|-
|colspan="1" style = "font-size:90%; color:black;" |CD5
|colspan="1" style = "background:#66e0ff;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:80%;"|Uncommon but seen in some cases
|-
|colspan="1" style = "font-size:90%;" |CD138
|colspan="1" style = "background:#006699;"|
|colspan="1" style = "font-size:80%;"|var
|colspan="1" style = "font-size:84%;"|Particularly on cells with plasma cell features
|-
|colspan="1" style = "font-size:90%; color:black;" |CD200
|colspan="1" style = "background:#66e0ff;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Detected infrequently
|-
|colspan="1" style = "font-size:90%; color:black;" |FMC7
|colspan="1" style = "background:#006699;"|
|colspan="1" style = "font-size:80%;"|
|colspan="1" style = "font-size:80%;"|Generally expressed
|-
!colspan="4" style = "font-size:90%; background:#DAE8F2"|Other relevant markers
|-
|colspan="1" style = "font-size:90%;" |CD10
|colspan="1" style = "background:#66e0ff;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Expression may be seen
|-
|colspan="1" style = "font-size:90%;" |CD11C
|colspan="1" style = "background:#006699;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|
|-
|colspan="1" style = "font-size:90%;" |CD20
|colspan="1" style = "background:#004466;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Expect expression
|-
|colspan="1" style = "font-size:90%; color:black;" |CD23
|colspan="1" style = "background:#00b8e6;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Infrequent but reported
|-
|colspan="1" style = "font-size:90%; color:black;" |CD25
|colspan="1" style = "background:#006699;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|
|-
|colspan="1" style = "font-size:90%;" |CD43
|colspan="1" style = "background:#66e0ff;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Generally not expressed by LPL
|-
|colspan="1" style = "font-size:90%; color:black;" |CD79b
|colspan="1" style = "background:#006699;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Generally expressed
|-
|colspan="1" style = "font-size:90%;" |CD103
|colspan="1" style = "background:#E6FAFF;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|
|-
|}
</div>

<span style="color:navy;font-size:80% ">'''Key to table: '''</span>

<span style="font-size:80% ">Key to colour code for expression frequency [[Key_to_marker_expression_table|Click for link]]</span>

<span style="font-size:80% ">Key to expression strength code and use [[Expression strength key|Click for link]]</span>

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