Flow cytometry: Marginal Zone Lymphoma - Revision history

John at 15:18, 13 July 2023

2023-07-13T15:18:21Z

← Older revision		Revision as of 17:18, 13 July 2023
Line 16:		Line 16:
	{\| class="wikitable" style="color:black; background-color:#ffffff;" cellpadding="5"		{\| class="wikitable" style="color:black; background-color:#ffffff;" cellpadding="5"
	!colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"\|'''Immunophenotype of MZL'''		!colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"\|'''Immunophenotype of MZL'''
			\|-
			!colspan="4" style = "font-size:90%; background:#DAE8F2"\|'''CD45''' expression
			\|-
			\|colspan="4" style = "font-size:90%; color:black;" \|Typically expression of CD45 approaches that of normal T and B cells.
	\|-		\|-
	!colspan="4" style = "font-size:90%; background:#DAE8F2"\|Major markers useful in diagnosis		!colspan="4" style = "font-size:90%; background:#DAE8F2"\|Major markers useful in diagnosis

John at 12:08, 13 July 2023

2023-07-13T12:08:27Z

← Older revision		Revision as of 14:08, 13 July 2023
Line 1:		Line 1:
	----		----
			<gallery mode="nolines" widths="200px" heights="200px" border="1px" >
	[[file:MZL.jpg~~\|200px~~\|link=MZL.jpg]]		file:MZL.jpg\|link={{filepath:MZL.jpg}}
			</gallery>
			----

John at 19:13, 12 July 2023

2023-07-12T19:13:22Z

← Older revision		Revision as of 21:13, 12 July 2023
Line 3:		Line 3:
	[[file:MZL.jpg\|200px\|link=MZL.jpg]]		[[file:MZL.jpg\|200px\|link=MZL.jpg]]

	<span style="font-size:90%">''Typical MZL morphology, note the small/medium size, bland chromatin pattern in a slightly squared nucleus, and the tendency of cytoplasm to gently engage adjacent red cells''</span>


Line 9:		Line 8:

	A difficult diagnosis - flow cytometry can suggest a diagnosis, but the immunophenotype may overlap with related diagnoses including atypical cases of CLL and MCL. Generally it is correct to suggest the diagnosis, but recognise that the profile is not fully specific, and confirmation by other modalities may be required.		A difficult diagnosis - flow cytometry can suggest a diagnosis, but the immunophenotype may overlap with related diagnoses including atypical cases of CLL and MCL. Generally it is correct to suggest the diagnosis, but recognise that the profile is not fully specific, and confirmation by other modalities may be required.

	~~'''CD45 Expression'''~~


	~~[[file:MZLCD45.jpg\|300px\|link=MZLCD45.jpg]]~~


	~~<span style="font-size:90%">''Side scatter''~~

John at 20:21, 4 July 2023

2023-07-04T20:21:44Z

← Older revision		Revision as of 22:21, 4 July 2023
Line 114:		Line 114:

	<span style="color:navy;font-size:80% ">'''Key to table: '''</span>		<span style="color:navy;font-size:80% ">'''Key to table: '''</span>

	~~<span style="font-size:80% ">Markers most useful in initial diagnosis are <span style=color:navy><u>underlined in blue text</u></span></span>~~

	<span style="font-size:80% ">Key to colour code for expression frequency [[Key_to_marker_expression_table\|Click for link]]</span>		<span style="font-size:80% ">Key to colour code for expression frequency [[Key_to_marker_expression_table\|Click for link]]</span>

John at 20:11, 4 July 2023

2023-07-04T20:11:12Z

John at 19:53, 4 July 2023

2023-07-04T19:53:53Z

← Older revision		Revision as of 21:53, 4 July 2023
Line 16:		Line 16:


	<span style="font-size:90%">''Side scatter of CLL cells is typically low, with a broad range and relatively weak expression of CD45 compared with normal lymphocytes. The CLL cells (highlighted in blue) therefore tend to the left of normal T cells (red) - giving the CLL population a rather elongated "slug-like" shape.'		<span style="font-size:90%">''Side scatter''

John at 17:09, 4 July 2023

2023-07-04T17:09:00Z

John at 17:07, 4 July 2023

2023-07-04T17:07:33Z

John at 09:40, 30 June 2023

2023-06-30T09:40:36Z

← Older revision		Revision as of 11:40, 30 June 2023
Line 60:		Line 60:
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-
	\|colspan="1" style = "font-size:84%;"\|Expression is expected, typically more strongly than in CLL		\|colspan="1" style = "font-size:84%;"\|Expression is expected, typically more strongly than in CLL
			\|-
			!colspan="4" style = "font-size:90%; background:#DAE8F2"\|Other relevant markers
	\|-		\|-
	\|colspan="1" style = "font-size:90%;" \|CD20		\|colspan="1" style = "font-size:90%;" \|CD20

John at 09:39, 30 June 2023

2023-06-30T09:39:07Z

← Older revision		Revision as of 11:39, 30 June 2023
Line 51:		Line 51:
	\|colspan="1" style = "font-size:84%;"\|Infrequently expressed, expression may suggest FL		\|colspan="1" style = "font-size:84%;"\|Infrequently expressed, expression may suggest FL
	\|-		\|-
	\|colspan="1" style = "font-size:90%;" \|CD11C		\|colspan="1" style = "font-size:90%;" \|'''CD11C'''
	\|colspan="1" style = "background:#006699;"\|		\|colspan="1" style = "background:#006699;"\|
	\|colspan="1" style = "font-size:80%;"\|		\|colspan="1" style = "font-size:80%;"\|
Line 86:		Line 86:
	\|colspan="1" style = "font-size:84%;"\|Lower expression than in CLL, may be helpful in some cases		\|colspan="1" style = "font-size:84%;"\|Lower expression than in CLL, may be helpful in some cases
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:navy;" \|<u>~~'''~~CD79b~~'''~~</u>		\|colspan="1" style = "font-size:90%; color:navy;" \|<u>CD79b</u>
	\|colspan="1" style = "background:#004466;"\|		\|colspan="1" style = "background:#004466;"\|
	\|colspan="1" style = "font-size:80%;"\|		\|colspan="1" style = "font-size:80%;"\|

← Older revision		Revision as of 11:39, 30 June 2023
Line 51:		Line 51:
	\|colspan="1" style = "font-size:84%;"\|Infrequently expressed, expression may suggest FL		\|colspan="1" style = "font-size:84%;"\|Infrequently expressed, expression may suggest FL
	\|-		\|-
	\|colspan="1" style = "font-size:90%;" \|CD11C		\|colspan="1" style = "font-size:90%;" \|'''CD11C'''
	\|colspan="1" style = "background:#006699;"\|		\|colspan="1" style = "background:#006699;"\|
	\|colspan="1" style = "font-size:80%;"\|		\|colspan="1" style = "font-size:80%;"\|
Line 86:		Line 86:
	\|colspan="1" style = "font-size:84%;"\|Lower expression than in CLL, may be helpful in some cases		\|colspan="1" style = "font-size:84%;"\|Lower expression than in CLL, may be helpful in some cases
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:navy;" \|<u>~~'''~~CD79b~~'''~~</u>		\|colspan="1" style = "font-size:90%; color:navy;" \|<u>CD79b</u>
	\|colspan="1" style = "background:#004466;"\|		\|colspan="1" style = "background:#004466;"\|
	\|colspan="1" style = "font-size:80%;"\|		\|colspan="1" style = "font-size:80%;"\|

← Older revision		Revision as of 22:11, 4 July 2023
Line 24:		Line 24:
	!colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"\|'''Immunophenotype of MZL'''		!colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"\|'''Immunophenotype of MZL'''
	\|-		\|-
	!colspan="4" style = "font-size:90%; background:#DAE8F2"\|~~'''~~Major markers useful in diagnosis~~'''~~		!colspan="4" style = "font-size:90%; background:#DAE8F2"\|Major markers useful in diagnosis
	\|-		\|-
	\|colspan="1" style = "font-size:80%; width: 4%; \|'''Marker'''		\|colspan="1" style = "font-size:80%; width: 4%; \|'''Marker'''
Line 31:		Line 31:
	\|colspan="1" style = "font-size:80%; width: 80%; \|'''Comment'''		\|colspan="1" style = "font-size:80%; width: 80%; \|'''Comment'''
	\|-		\|-
	\|colspan="1" style = "width: 4%; font-size:90%; color:black;" \|~~'''~~κ/λ~~'''~~		\|colspan="1" style = "width: 4%; font-size:90%; color:black;" \|κ/λ
	\|colspan="1" style = "width: 8%; background:#004466;"\|		\|colspan="1" style = "width: 8%; background:#004466;"\|
	\|colspan="1" style = "font-size:80%; width: 8%; \|		\|colspan="1" style = "font-size:80%; width: 8%; \|
	\|colspan="1" style = "font-size:80%;"\|Expect moderate or strong restricted κ or λ expression		\|colspan="1" style = "font-size:80%;"\|Expect moderate or strong restricted κ or λ expression
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:black;" \|~~'''~~FMC7~~'''~~		\|colspan="1" style = "font-size:90%; color:black;" \|FMC7
	\|colspan="1" style = "background:#006699;"\|		\|colspan="1" style = "background:#006699;"\|
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-
	\|colspan="1" style = "font-size:80%;"\|FMC7 may be expressed in MZL and may be strong		\|colspan="1" style = "font-size:80%;"\|FMC7 may be expressed in MZL and may be strong
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:black;" \|~~'''~~CD5~~'''~~		\|colspan="1" style = "font-size:90%; color:black;" \|CD5
	\|colspan="1" style = "background:#66e0ff;"\|		\|colspan="1" style = "background:#66e0ff;"\|
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-
	\|colspan="1" style = "font-size:80%;"\|Expression of CD5 is uncommon in MZL		\|colspan="1" style = "font-size:80%;"\|Expression of CD5 is uncommon in MZL
	\|-		\|-
	\|colspan="1" style = "font-size:90%;" \|~~'''~~CD10~~'''~~		\|colspan="1" style = "font-size:90%;" \|CD10
	\|colspan="1" style = "background:#66e0ff;"\|		\|colspan="1" style = "background:#66e0ff;"\|
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-
	\|colspan="1" style = "font-size:84%;"\|Infrequently expressed, expression may suggest FL		\|colspan="1" style = "font-size:84%;"\|Infrequently expressed, expression may suggest FL
	\|-		\|-
	\|colspan="1" style = "font-size:90%;" \|~~'''~~CD11C~~'''~~		\|colspan="1" style = "font-size:90%;" \|CD11C
	\|colspan="1" style = "background:#006699;"\|		\|colspan="1" style = "background:#006699;"\|
	\|colspan="1" style = "font-size:80%;"\|		\|colspan="1" style = "font-size:80%;"\|
	\|colspan="1" style = "font-size:84%;"\|Frequently expressed in MZL and should be looked for		\|colspan="1" style = "font-size:84%;"\|Frequently expressed in MZL and should be looked for
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:black;" \|~~'''~~CD19~~'''~~		\|colspan="1" style = "font-size:90%; color:black;" \|CD19
	\|colspan="1" style = "background:#004466;"\|		\|colspan="1" style = "background:#004466;"\|
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-

← Older revision		Revision as of 19:09, 4 July 2023
Line 46:		Line 46:
	\|colspan="1" style = "font-size:80%;"\|Expression of CD5 is uncommon in MZL		\|colspan="1" style = "font-size:80%;"\|Expression of CD5 is uncommon in MZL
	\|-		\|-
	\|colspan="1" style = "font-size:90%;" \|CD10		\|colspan="1" style = "font-size:90%;" \|'''CD10'''
	\|colspan="1" style = "background:#66e0ff;"\|		\|colspan="1" style = "background:#66e0ff;"\|
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-
Line 68:		Line 68:
	\|colspan="1" style = "font-size:84%;"\|Expression is expected, typically stronger than CLL		\|colspan="1" style = "font-size:84%;"\|Expression is expected, typically stronger than CLL
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:black;" \|~~'''~~CD23~~'''~~		\|colspan="1" style = "font-size:90%; color:black;" \|CD23
	\|colspan="1" style = "background:#66e0ff;"\|		\|colspan="1" style = "background:#66e0ff;"\|
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-
Line 103:		Line 103:
	\|colspan="1" style = "font-size:84%;"\|Expression may occur in MZL		\|colspan="1" style = "font-size:84%;"\|Expression may occur in MZL
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:black;" \|~~'''~~CD200~~'''~~		\|colspan="1" style = "font-size:90%; color:black;" \|CD200
	\|colspan="1" style = "background:#66e0ff;"\|		\|colspan="1" style = "background:#66e0ff;"\|
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-

← Older revision		Revision as of 19:07, 4 July 2023
Line 31:		Line 31:
	\|colspan="1" style = "font-size:80%; width: 80%; \|'''Comment'''		\|colspan="1" style = "font-size:80%; width: 80%; \|'''Comment'''
	\|-		\|-
	\|colspan="1" style = "width: 4%; font-size:90%; color:~~navy~~;" \|~~<u>~~'''κ/λ'''~~</u>~~		\|colspan="1" style = "width: 4%; font-size:90%; color:black;" \|'''κ/λ'''
	\|colspan="1" style = "width: 8%; background:#004466;"\|		\|colspan="1" style = "width: 8%; background:#004466;"\|
	\|colspan="1" style = "font-size:80%; width: 8%; \|		\|colspan="1" style = "font-size:80%; width: 8%; \|
	\|colspan="1" style = "font-size:80%;"\|Expect moderate or strong restricted κ or λ expression		\|colspan="1" style = "font-size:80%;"\|Expect moderate or strong restricted κ or λ expression
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:~~navy~~;" \|~~<u>~~'''FMC7'''~~</u>~~		\|colspan="1" style = "font-size:90%; color:black;" \|'''FMC7'''
	\|colspan="1" style = "background:#006699;"\|		\|colspan="1" style = "background:#006699;"\|
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-
	\|colspan="1" style = "font-size:80%;"\|FMC7 may be expressed in MZL and may be strong		\|colspan="1" style = "font-size:80%;"\|FMC7 may be expressed in MZL and may be strong
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:~~navy~~;" \|~~<u>~~'''CD5'''~~</u>~~		\|colspan="1" style = "font-size:90%; color:black;" \|'''CD5'''
	\|colspan="1" style = "background:#66e0ff;"\|		\|colspan="1" style = "background:#66e0ff;"\|
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-
Line 56:		Line 56:
	\|colspan="1" style = "font-size:84%;"\|Frequently expressed in MZL and should be looked for		\|colspan="1" style = "font-size:84%;"\|Frequently expressed in MZL and should be looked for
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:~~navy~~;" \|~~<u>~~'''CD19'''~~</u>~~		\|colspan="1" style = "font-size:90%; color:black;" \|'''CD19'''
	\|colspan="1" style = "background:#004466;"\|		\|colspan="1" style = "background:#004466;"\|
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-
Line 68:		Line 68:
	\|colspan="1" style = "font-size:84%;"\|Expression is expected, typically stronger than CLL		\|colspan="1" style = "font-size:84%;"\|Expression is expected, typically stronger than CLL
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:~~navy~~;" \|~~<u>~~'''CD23'''~~</u>~~		\|colspan="1" style = "font-size:90%; color:black;" \|'''CD23'''
	\|colspan="1" style = "background:#66e0ff;"\|		\|colspan="1" style = "background:#66e0ff;"\|
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-
Line 88:		Line 88:
	\|colspan="1" style = "font-size:84%;"\|Lower expression than in CLL, may be helpful in some cases		\|colspan="1" style = "font-size:84%;"\|Lower expression than in CLL, may be helpful in some cases
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:~~navy~~;" \|~~<u>~~CD79b~~</u>~~		\|colspan="1" style = "font-size:90%; color:black;" \|CD79b
	\|colspan="1" style = "background:#004466;"\|		\|colspan="1" style = "background:#004466;"\|
	\|colspan="1" style = "font-size:80%;"\|		\|colspan="1" style = "font-size:80%;"\|
Line 103:		Line 103:
	\|colspan="1" style = "font-size:84%;"\|Expression may occur in MZL		\|colspan="1" style = "font-size:84%;"\|Expression may occur in MZL
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:~~navy~~;" \|~~<u>~~'''CD200'''~~</u>~~		\|colspan="1" style = "font-size:90%; color:black;" \|'''CD200'''
	\|colspan="1" style = "background:#66e0ff;"\|		\|colspan="1" style = "background:#66e0ff;"\|
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-